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Entomopathogenic nematodes (Heterorhabditis bacteriophora)


Use Larvanem for:

Larvae of various species of beetle such as the black vine weevil (Otiorhynchus sulcatus)


Each box contains nematodes (third stage) in gel

Standard Larvanem: 2 bags in a box

Each bag contains: 25 million nematodes

Large Larvanem: 2 bags in a box

Each bag contains: 250 million nematodes 

General information

When to use Larvanem?

Use Larvanem for biological pest control of the larvae of various species of beetles, such as the black vine weevil (Otiorhynchus sulcatus).

How does Larvanem work?

After application, the nematodes actively search for their prey and penetrate them. The nematodes feed on the contents of the prey, excreting specific bacteria from their digestive tracts as they do so. These bacteria convert host tissue into products that the nematodes can easily ingest. The larvae die within a few days. 

How to use Larvanem

Application Larvanem

Preparation of the spray solution

  1. Half an hour before preparing the product, remove the pack from the refrigerator and let it reach room temperature (20°C/68°F)

  2. Break the contents of the sachet (250 million Heterorhabditis bacteriophora) into smaller pieces

  3. Use the entire pack and place the smaller pieces in a bucket containing five liters of water (at 15°C/59°F to 20°C/68°F)

  4. Leave the bucket with the solution to stand for five minutes

  5. Then stir the solution until all the lumps have disappeared before transferring it to the spray tank

  6. Use the spray solution immediately after preparation


  1. With a watering can, sprinkler system, backpack spray unit or vehicle-mounted spray unit

  2. To avoid blockage, all filters should be removed

  3. Use a maximum pressure of 12 bar on spraying boom or pistol

  4. The spray nozzle opening should be at least 0.5 mm (500 microns)

  5. Continuous mixing should take place to prevent nematodes sinking to the bottom of the spray tank

Soil application

  1. Dosage of 0.5 million nematodes/m²

  2. Amount of spray solution depends on application method

  3. Water the soil before and after application

  4. Distribute the spraying solution evenly over the soil surface

  5. Keep the soil moist for the first two weeks after application

Leaf application

  1. Use 0.25 million nematodes/m² mixed into 0.125 litre spray solution (=1250 litres/hectare)

  2. A wetting agent will improve efficacy

  3. Spray the crop evenly with the Entonem solution

  4. Repeat treatment every week as long as necessary


Best working conditions Larvanem

  • The moisture content of the soil must be high

  • Soil temperature between 14 and 33°C

  • Nematodes are susceptible to ultraviolet light (UV): do not use them in direct sunlight

  • Leaf applications should only be carried out in the evening to minimise the influence of UV light and to maximize moisture availability for the nematodes


Biological beneficials have a very short life expectancy and therefore need to be introduced into the crop as soon as possible after receipt. Failure to do so can have a negative impact on their quality. In case you do need to store Larvanem, please follow the instructions below. Shale Peak Horticulture, LLC is not liable for any loss of quality if the product is stored for longer than recommended and/or under incorrect conditions.

Storage instructions:

  • Shelf life: see package for expiry date

  • Storage temperature: 2-6 °C

  • Store in a dark place


The information given below is merely indicative. Tailored advice can be provided if information is available on the local factors that need to be taken into account, such as the crop, the climate conditions and the level of infestation. For the correct approach, please consult a Shale Peak Horticulture specialist.


  • Only use products that are permitted in your country/state and crop. Check local registration requirements.

  • Shale Peak Horticulture, LLC  cannot be held liable for unauthorized use.

  • Shale Peak Horticulture, LLC  is not liable for any loss of quality if the product is stored for longer than recommended and/or under incorrect conditions.

Side effects

Pesticides can have (in)direct effects on natural enemies. Koppert’s side effects app tells you how harmful different pesticides are.

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